Composite

Part:BBa_K1480003

Designed by: Jesus Gilberto Rodriguez Ceja   Group: iGEM14_UANL_Mty-Mexico   (2014-10-14)

TetR generator (Promoter J23109)

This is a TetR protein generator regulated by the constitutive promoter J23109.

The protein has a LVA tag for degradation.

Usage and Biology

It can be used as a constitutive generator of TetR to prove the function of a gene (or sequence) regulated by tet promoter.

Results

It is shown the effect of 4 different TetR generators on a mCherry gene (K1140003) that is down regulated by ptet. To the right side are listed the names of the generators.

Here are the links for the other parts used.

https://parts.igem.org/Part:BBa_K1140006
https://parts.igem.org/Part:BBa_K1480003
https://parts.igem.org/Part:BBa_K145201
https://parts.igem.org/Part:BBa_K1480004
https://parts.igem.org/Part:BBa_K1480005


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/repressor
//classic/generator/uncategorized
//direction/forward
//function/regulation/transcriptional
Parameters
directionForward
familyTetR
functionRepressor
ligandsATc / Tetracycline
proteinTetR repressor
tagLVA
targetptet